Composition of the Kit
| Reagent |
Specification |
Microtiter Plate
|
96T×2 |
| Antibody solution (blue cap) |
1×11mL |
| Enzyme conjugate(reg cap) |
2×11mL |
| Substrate Reagent A(white cap) |
1×11mL |
| Substrate Reagent B (brown cap) |
1×11mL |
| Stop Solution (yellow cap) |
1×11mL |
| Concentrated Wash Buffer (20×)(white cap) |
1×40mL |
| Negative control(green cap) |
1×1.5mL |
| Positive control(red cap) |
1×1.5mL |
| Instructions |
1 |
| Adhesive Membrane |
2 |
| Sealed bag |
1 |
Avian influenza, also known as highly pathogenic avian influenza (HPAI), fowl plague, or European fowl plague, is an acute and highly lethal contagious disease affecting birds, caused by the Avian Influenza Virus (AIV) of the A type. It is characterized by acute septicemic death or asymptomatic carrier status.
Intended Use
This reagent is used for the detection of antibodies against the AIV in the serum, plasma, or egg yolk of poultry such as chickens, ducks, and geese. It can be employed for evaluating immune responses and assisting in diagnostic processes.
Principle
This assay kit comprises a coated Microtiter Plate (ELISA) plate with avian influenza virus antigen, antibody solution, Enzyme conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the AIV in the serum, plasma, or egg yolk of avian species. During the experiment, diluted samples and antibody solution are added to the plate. If the sample contains avian influenza virus antibodies, they will compete with the antibody solution to bind with the antigen on the ELISA plate, thereby preventing subsequent color development. Conversely, color development occurs in the absence of such antibodies. The intensity of the color is inversely proportional to the specific antibody content in the sample. After adding a stop solution to terminate the reaction, the product turns yellow. The absorbance values in each well are measured at a wavelength of 450nm using an microtiter plate reader（ELISA reader）, allowing determination of the presence of AIV antibodies in the sample.
About us
Shenzhen Finder Biotech Co., Ltd. is a a high-tech enterprise located in Shenzhen, China. Our company is engaged in rapid detection of food safety (such as residue detection of veterinary drugs, additives, hormones, mycotoxins) , rapid diagnosis of animal diseases and related product development.There are hundreds of products. Secondly, the diagnostic kits and test cards for animal diseases mainly for livestock have been widely used in Animal Husbandry Government Department, scientific research institutions and universities, which provides a strong basis and guarantee for monitoring immunity and diagnosis.
