Product: 2*Taq PCR Master (without dye)

Cat. No.: T0204

Price: $5/ml  (negotiable if bulk purchase)

Storage conditions: -20℃

Product description

The concentration of Taq-ASPCRMix is ​​2×. It is easy and quick to use and can reduce contamination during PCR operation. When using, just take an appropriate amount of 2×Taq-AS PCR Mix, add template and primers, and add ddH2O to make up the volume, so that the concentration of the reaction system is 1×, and then you can carry out PCR reaction. The 3' end of the PCR product has a prominent A base and can be directly used for T/A cloning after purification.

The TaqDNA polymerase in this Mix is ​​a screened Taq-AS (Advanced Strong) DNA Polymerase mutant, which can efficiently amplify DNA fragments ≤5kb and has good inhibitor tolerance. Its amplification speed is about 3 times that of ordinary Taq DNA polymerase, so it can greatly reduce the time required for the PCR extension process, thereby shortening the entire PCR reaction. Different from the fast Taq polymerase based on the fusion protein principle, the use of Taq-AS is closer to WT-Taq, and it is not easy to have electrophoretic band diffusion, dragging or fragment size changes.

Quality control

Nuclease activity detection

25μl 2×Taq-AS PCR Mix and 200ng supercoiled plasmid DNA were prepared into a 50μl reaction system. After incubation at 37℃ for 4h, less than 10% of the plasmid DNA was converted into a nicked or linear state by agarose gel electrophoresis.

Nonspecific nuclease activity detection

25μl 2×Taq-AS PCR Mix and 15ng double-stranded DNA fragment were prepared into a 50μl reaction system. After incubation at 37℃ for 16h, agarose gel electrophoresis was used to detect that there was no change in the double-stranded DNA substrate.